FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24064568
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC781920.3249258411786158No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT530620.22049845233041376No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA392160.1629615790318779No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA366740.1523983310234366No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT360190.14967648702440867No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA353940.14707930763602323No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT279780.11626221588519686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC406450.0115.564741
CGGGTGC121150.0115.2364351
CGGTTAA896250.0112.762111
CGGGTAC142150.0111.751721
CGGCTAA7750.0108.407261
GGGCGCG486950.0106.251222
CGGATGC13850.0101.962271
GGCGCGG503300.099.341993
CGGGTAT497050.098.767791
GCGCGGT498350.098.717274
CGCGGTG508100.098.23965
CGGGTGT424100.095.8763961
GGGAGGC720800.095.717692
CGGGAGG1478000.095.316721
CGGATAC15500.094.952451
CGGGTTT742050.094.3829351
CGGAATA161950.093.563481
CGGTAAT151850.092.3311161
CGGGCGT656050.092.2052461
CGGTGGT875250.092.052787
Produced by FastQC (version 0.11.5)