FastQCFastQC Report
Thu 12 May 2022
EGAF00005416830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25098815
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC560950.22349660730994672No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT499400.19897353719687563No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA336180.13394257856396807No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT320320.12762355513596957TruSeq Adapter, Index 18 (97% over 40bp)
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA299760.1194319333402792No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT274630.10941950845089697No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA273940.10914459507351244No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT272550.10859078406689718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)