FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22150614
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT565240.25518028529592907No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA530340.23942451437237813No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA461170.20819738902045784No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC394240.17798152231807207No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT363250.16399093948366397No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA295620.13345905445329867No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT294690.13303920153184015No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG247730.11183888627195616No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA246030.11107141318972015No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT225850.10196105624882452No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT222130.10028164456299044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC103150.0110.098511
CGGCTAA8050.0106.584571
CGGTTAA667650.0106.2538761
CGGGCGC240000.0104.445331
CGGGTAC99550.0102.6480261
GGGAGGC679350.098.508722
CGGGAGG1571050.096.438391
CGGAATA272350.093.395631
CGGGTGT371400.093.306151
CGGGTAA123650.092.134341
CGGGTAT400850.091.5792541
CGGTAAT150800.091.035111
GGGCGCG315750.090.8231662
CGGGTTT802800.090.266251
CGGGAAG222600.089.482681
CGGGATA108750.087.992171
GCGGAGT432800.086.784787
CGGTATT364100.085.7999341
CGGAATG1293550.085.3440251
CGGGAAA94800.084.975971
Produced by FastQC (version 0.11.5)