FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20808806
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC527000.2532581638754285No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT335050.16101356320011828No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA251480.12085268131194073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC121850.0113.1860351
CGGGCGC303200.0110.000941
CGGTTAA716850.0108.31771
CGGGTAC129550.0107.7899551
CGGCTAA8350.0105.412681
GGGCGCG363350.097.468892
GGGAGGC646700.096.083572
CGGGTGT410200.094.834441
CGGGTAT449750.094.0852051
CGGGAGG1511650.092.593521
CGGAATA212200.091.226971
CGGTAAT157350.091.2028051
CGGGTTT811300.088.985461
CGCGGTG379050.088.9483265
CGGGTAA124300.088.850191
GGCGCGG384900.088.769723
CGGGCGT578900.088.495041
CGGATGC12150.088.107691
GCGCGGT379550.088.0796664
GCGGAGT429950.086.282637
Produced by FastQC (version 0.11.5)