FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22328154
Sequences flagged as poor quality0
Sequence length125
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT722540.3236004194524993No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA554110.24816650762978434No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC443980.19884312872438986No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT442050.1979787491612607No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA407320.1824243956755225No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT275980.12360179887687985No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT234690.10510945060661979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC100300.0111.3299561
CGGTTAA794050.0111.251841
CGGCTAA12350.0110.465371
CGGGCGC259150.0106.1211851
CGGGTAC106350.0104.827131
GGGAGGC617850.097.517082
CGGGAGG1408450.096.865881
CGGAATA310050.095.9490661
CGGATGC10800.094.600291
CGGGTGT321200.093.4977341
CGGTAAT147500.093.26551
CGGGTAA109700.091.874231
GGGCGCG326750.091.728432
CGGGTAT343450.091.307791
GCTAATT14350.090.836193
CGGAGTG142700.089.916871
CGGATGA69000.089.01621
CGGGTTT690600.088.9388661
CGGGAAG204350.088.641251
GCGGAGT397900.087.95447
Produced by FastQC (version 0.11.5)