FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16383928
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT608740.371547042931341No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA466380.2846570126528876No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA464510.28351565021525976No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT449470.27433592237465887No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT378530.23103739225416517No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT331070.20206997979971592No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC326800.19946376717475808No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA312920.19099205025803334No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA256370.156476517719072No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA248270.15153264833683353No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT217410.13269711634474957No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA213600.1303716666723633No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA206370.12595880548303193No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA203240.12404839669705581No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAA177330.10823411821633983No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA175700.1072392407974449No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT174580.10655564404335761No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAG167540.10225875016052317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA9850.0116.006081
CGGGTGC77850.0111.382421
CGGTTAA636100.0110.410191
CGGGCGC187350.0106.225391
CGGGTAC77700.0101.563641
CGGAATA214550.097.9733351
CGGGTGT245000.095.294521
GGGAGGC395800.094.914592
CGGTAAT104350.094.845231
CGGGAGG918400.094.823781
CGGGTAA75700.091.51091
GGGCGCG238650.091.408152
CGGGTAT262650.090.612851
CGGGTTT529850.089.801021
CGGGAAG143900.089.208261
CGGATGC7800.088.507311
CGGAATT180900.086.918051
CGGTATT246700.086.122131
GCTAATT13150.085.950033
CGGAGTA105400.085.9388961
Produced by FastQC (version 0.11.5)