FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16338114
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT570160.34897540805505456No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA409650.2507327345126861No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC348850.21351913690894803No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT337470.2065538286732483No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT272240.16662877979673785No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA257200.15742331091581319No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA201030.12304357773485974No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT195120.11942626915199636No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA187960.11504387838155616No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA184200.112742511161325No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT183960.11259561538131023No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT183010.11201415291875183No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA182470.11168363741371863No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT171500.10496927613554416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA10250.0112.0886461
CGGTTAA766600.0111.906031
CGGGTGC82250.0111.2413251
CGGGCGC193800.0105.020361
CGGGTAC79450.0103.323371
CGGGAGG991900.097.314431
GGGAGGC431500.097.2118762
CGGACTT800.096.7345351
CGGAATA238750.095.4208451
CGGGTGT259050.094.814291
CGGTCTA1450.094.4252241
CGGTAAT112400.092.36521
GCTAATT13300.091.703473
CGGGTAT270050.091.238771
CGGGTAA82700.091.2009351
CGGGTTT622500.091.0195161
GGGCGCG245250.089.461622
CGGATGC9050.089.4578551
CGGATGA60400.089.09631
CGGGAAG160250.088.559751
Produced by FastQC (version 0.11.5)