FastQCFastQC Report
Wed 11 May 2022
EGAF00005416946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25277605
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1021060.40393858516263703No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA796900.3152592977064085No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC655590.25935605845569626No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT626180.24772125365516234No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT613020.24251506422384556TruSeq Adapter, Index 14 (97% over 44bp)
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA544340.2153447686202866No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT496770.1965257388902153No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA403320.15955625542847116No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT387570.1533254436090761No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA322180.1274566953633463No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT297810.11781575034501884No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT293380.11606321089359534No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT289360.11447287035302592No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA266870.1055756666820294No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA264140.10449565930000092No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA256390.10142970427775891No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA253620.10033387261174466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)