FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005418171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23531746
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC768020.3263761218568312No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT642050.2728441824928758No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA618150.2626876900677068No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT519320.220689106537186No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA483930.2056498485067789No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT345220.14670394623501376No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT311360.1323148737029543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT309450.1315032042246249TruSeq Adapter, Index 14 (97% over 44bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT294710.12523932563270063No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA282010.11984236103857317No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA236030.10030279946077948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)