FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22344691
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC782440.35016818983981474No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT486230.2176042622383993No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT343600.15377254489668263TruSeq Adapter, Index 14 (97% over 44bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT292440.1308767259301102No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT282050.12622685182802484No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA260750.11669438615194991No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA248250.11110021615425338No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA226080.10117839624633879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC381450.0114.417331
CGGTTAA1678400.0114.239851
CGGGTGC103650.0112.168011
CGGCTAA11850.0109.6274261
CGGGTAC142250.0105.860521
GGGCGCG455750.0101.330522
CGGAATA257550.098.450761
GGCGCGG469150.095.649323
CGCGGTG470200.094.878955
GCGCGGT470700.093.185474
CGGGTGT339300.091.4326551
CGGGTAT392150.090.9023741
CGGATAA124150.090.4305651
CGGGCGT542000.090.321121
CGGGTTT1085950.089.7912751
CGGTAAT140300.089.662661
CGGATAC18650.089.466461
CGGAGTG137600.088.477131
CGGAATT275550.088.1268541
CGGTGGT813300.087.598287
Produced by FastQC (version 0.11.5)