FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18232346
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC371230.2036106598679073No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT259300.14221976700091146No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA237660.13035075135147173No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA233160.12788261038924997No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA208110.11414329236621552No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT192100.10536219529839988TruSeq Adapter, Index 16 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC108350.0113.266981
CGGGCGC225400.0108.0186161
CGGTTAA528150.0107.1836551
CGGGTAC92500.0105.946181
CGGCTAA5700.0101.876791
CGGGGAC38800.096.7418061
CGGGTCT6950.095.6129151
CGGGTAA116800.094.3603061
CGGGTCA2800.094.0747451
CGGGTGT348550.094.071051
GGGCGCG277900.093.578232
CGGGCTT6850.092.638961
CGGGCAT3950.092.450891
CGGGTCC3150.091.2241
GGGAGGC394350.091.038652
CGGGTAT326350.090.913011
CGGAATA204850.090.741091
CGGATGC13150.090.140051
CGGATTG134950.090.098051
GGTTACT14900.089.998512
Produced by FastQC (version 0.11.5)