FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22065610
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC843700.3823596990973737No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT553470.25082923155081593No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA387470.17559904303574658No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA323750.14672152729972116No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT303220.13741745639481528No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA230980.10467872857355857No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT229840.10416208751990089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC428900.0113.859661
CGGGTGC131700.0112.27261
CGGTTAA917150.0110.049961
CGGGTAC162500.0108.310691
GGGCGCG499200.0103.850972
CGGCTAA7750.099.93451
CGGGTAT515250.097.279491
GGCGCGG520000.096.581753
CGCGGTG517500.096.105895
GCGCGGT514400.095.7023544
CGGGTGT421750.094.776731
CGGGCGT652500.093.181011
CGGTAAT157650.092.4793851
CGGAATA269950.092.356221
CGGGTTT901950.090.9881
GGGAGGC542750.090.572722
CGGGTAA115100.089.838051
CGGGGAC30700.089.7415161
CGGATGC12700.089.3806151
GCGGTGG745050.089.343426
Produced by FastQC (version 0.11.5)