FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19384940
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT628270.32410211225827884No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC520250.2683784422340229No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA504300.26015040541781403No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA442810.22842990486429157No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT387610.19995419124330538No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT353320.18226520174939928TruSeq Adapter, Index 15 (97% over 40bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA312770.16134690125427265No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT263800.13608502270319123No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA256370.13225215038065632No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT254950.131519622965044No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA220930.11396991685297968No Hit
CGGGGACGCGGAAGTTTGTTTGAGGGAGGAGGGGTGGAAGGAGAGATAGT212920.10983784319167353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC108350.0110.401321
CGGTTAA652600.0107.859551
CGGCTAA7500.0106.487661
CGGGCGC290550.0103.838181
CGGGTAC121500.0101.690141
CGGAATA305500.095.030431
CGGTAAT146800.094.31461
CGGGTGT390650.093.662461
GGGAGGC566850.093.219652
CGGGTAT434200.090.9668051
CGGGTAA118850.090.76851
CGGGAGG1441600.089.116691
CGGGTTT854400.088.1323551
CGGGGAC47350.087.985881
GGGCGCG362750.086.9457242
CGGTATT367450.085.740241
CGGAGTG124250.085.7203451
CGGTATA112650.084.917971
CGGGATA112700.084.351451
CGGGAAG220850.083.5524751
Produced by FastQC (version 0.11.5)