FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13172813
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC564390.42845062781958565No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT478780.3634607126055764No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA346800.2632695081908473No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA343610.26084785383349784No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT272520.20688064121156202TruSeq Adapter, Index 18 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT257370.19537968086239438No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA191980.14573956223321471No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG191610.14545868069333406No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT168320.12777832646679185No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA148620.11282328231638906No Hit
TGGTTAGTGGGGGTAGGTTTTTTAGGGAGAGGAGGGTGGATGGAATTAAG146550.11125186397165131No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT143460.1089061235439993No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA141060.10708418923125987No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT139500.10589993192797924No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT136470.10359973985814572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC274700.0113.386671
CGGGTGC90200.0112.991431
CGGTTAA618950.0110.991261
CGGGTAC108350.0108.247741
CGGCTAA4950.0104.692631
GGGCGCG321800.0101.148462
CGGGTAT305750.097.7416461
CGGGTGT274150.095.775711
CGCGGTG325950.095.435625
GGCGCGG334100.094.015683
GCGCGGT327350.093.918914
CGGGCGT389350.093.323641
GGGAGGC393800.092.478782
CGGGAGG926400.092.371791
CGGGTTT569650.092.1338351
CGGAATA166200.091.5359951
CGGTAAT88150.089.738291
CGGTGGT579900.089.473477
GCGGTGG478100.087.757956
CGGAAAT71650.087.707811
Produced by FastQC (version 0.11.5)