FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19236920
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT267250.13892556604695555No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC238940.12420907297010124No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA202890.10546906677368309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA4900.0194.878661
CGGGTGC61850.0188.5281
CGGTTAA408200.0182.569981
CGGGCGC148650.0179.368181
CGGGTAC58900.0168.60811
CGGAATA188900.0147.552291
CGGTACT650.0146.908541
CGGGCAT2400.0145.888341
GGGCGCG204200.0142.189652
CGGGTGT227650.0141.451931
GGGAGGC287300.0140.091132
CGGTAAT94400.0136.784361
CGGGTAA69250.0134.062131
CGGGTTT485950.0133.490921
CGGGTAT233800.0133.056871
CGGGAGG736400.0131.372251
CGGAGTG77900.0126.1221541
CGGTATT225200.0125.935581
CGGATGC8350.0125.795931
CGGAGTA98450.0124.583341
Produced by FastQC (version 0.11.5)