FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21872602
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT550270.25157957887223475No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC511890.23403251245553686No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA428740.19601691650586428No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT397750.18184850618138618No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT303780.1388860822320088No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA292490.1337243735336107No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA281500.1286998227279955No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA232060.1060962019973664No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT223760.10230150029703827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC118300.0113.3004151
CGGGCGC280400.0109.908661
CGGTTAA751550.0109.730631
CGGCTAA7150.0107.382011
CGGGTAC114300.0106.5907441
GGGCGCG341700.096.860972
GGGAGGC598350.095.549452
CGGGAGG1399550.094.970131
CGGGTGT380050.094.511451
CGGGTAT382100.093.910921
CGGATGC10500.093.528191
CGGAATA288350.091.5628741
CGGTAAT141650.091.0521551
CGGGTTT729800.090.7367251
CGGGTAA118900.089.552221
GGCGCGG362800.088.034983
CGGGCGT495700.087.589951
GCGGAGT399150.087.523837
GCGCGGT359650.087.116954
CGGGCAT3700.086.864011
Produced by FastQC (version 0.11.5)