FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15372151
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC524730.3413510575065259No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT309790.20152677396936838No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC268490.1746600069177046TruSeq Adapter, Index 4 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT216870.1410798007383612No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT180180.11721196337454662No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA173330.1127558531008445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC269300.0113.6215741
CGGTTAA1090700.0113.560481
CGGGTGC77700.0111.313021
CGGCTAA8650.0108.504211
CGGGTAC103450.0106.861561
GGGCGCG323400.0100.7850952
GGCGCGG332300.095.146053
CGCGGTG336750.094.348565
CGGAATA167200.093.5804441
GCGCGGT333550.093.1348954
CGGGTGT259100.092.325111
CGGGTAT293500.092.089251
CGGGTTT777900.090.917541
CGGGCGT394850.090.4315951
CGGGCAT2300.090.395291
CGGAATT186750.089.1277851
CGGTAAT104500.087.8817751
CGGAGTG94500.087.438141
CGGTGGT591700.087.173177
GCGGTGG479900.086.8372966
Produced by FastQC (version 0.11.5)