FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005418329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21128473
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC641330.30353826327155775No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA416190.19698063366907775No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT390160.18466076559342456No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG354430.1677499363063294No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT347590.16451259870980736No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA328640.15554365902353665No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC295370.13979713536326077TruSeq Adapter, Index 12 (100% over 50bp)
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA289660.13709462108312323No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA283920.13437790795387816No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT239510.11335887832499775No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT222570.10534126152893303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)