FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16402583
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC461610.2814251877280548No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT353340.21541729128881715No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA262300.15991383796076508No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT214280.1306379611064916No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA212920.12980882340299696No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG203660.1241633710983203No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT203410.12401095607929556No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA177480.10820247030604874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1195700.0114.268551
CGGGCGC219950.0114.2569051
CGGGTGC73600.0112.038661
CGGGTAC92900.0107.39891
CGGCTAA9100.0101.9919051
GGGCGCG264650.099.345232
CGGAATA184700.095.7336041
CGCGGTG274750.093.329645
GGCGCGG275350.092.979353
CGGGTTT861100.091.692241
CGGGTAT304150.091.546241
GCGCGGT276450.091.013324
CGGGTGT275050.090.870631
CGGAATT199100.087.913161
CGGGCGT358550.087.861521
CGGAGTG100600.087.586981
CGGGTAA80450.087.0428161
CGGATAC14600.086.79791
CGGAGTA127250.086.262311
CGGTGGT512050.086.244347
Produced by FastQC (version 0.11.5)