FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234370
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC426130.279716194368392No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT392210.25745075116332344TruSeq Adapter, Index 15 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT308120.20225319458566388No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT218900.14368825228742638No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA183040.12014937276697361No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT175010.11487839667803788No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA169590.11132065192062422No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA168980.11092024153279723No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT163370.10723777878573251No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA162760.10683736839790553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1058400.0114.512181
CGGGCGC205300.0113.7374041
CGGGTGC60900.0110.639641
CGGGTAC76450.0105.918511
GGGCGCG245100.0102.019352
CGGCTAA6200.0100.982751
GGCGCGG254900.094.907313
CGCGGTG255250.094.264365
CGGAATA156200.093.297451
CGGGCAT1950.091.7352451
GCGCGGT257950.091.6861344
CGGGTTT818500.090.8150941
CGGGTAT262500.089.248791
CGGAAAT79200.088.839561
CGGAGTG92900.088.639551
CGGGCGT352600.088.1735461
CGGAATT179100.087.760571
CGGGTGT236550.087.6206361
CGGATAA68950.087.6041641
CGGTAAT86400.086.336241
Produced by FastQC (version 0.11.5)