FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19937763
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA386930.19406891334800197No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT381090.19113979838159376No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA334360.16770186304250884No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC288140.1445197237022027No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT251440.1261124430057675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC83400.0111.905691
CGGCTAA5200.0108.9492341
CGGTTAA488800.0105.557531
CGGGCGC178850.0104.966251
CGGGCTT6400.0101.566481
CGGGTAC70750.0100.979751
CGGGCAT3900.0100.921391
CGGGGAC31700.097.260211
GGGAGGC448700.097.023312
CGGGTAA123400.096.0255051
CGGATTG141050.096.016951
CGGAATA308900.095.737061
CGGGAGG1005550.093.709751
CGGGTGT299850.092.878821
GGGTGGC100750.091.488152
GGGCGCG230800.091.217992
GCGGAGT349850.090.6287
CGGGCTA1600.089.453051
CGGCTAT800.089.453051
ATTGCCA208.4805075E-489.258313
Produced by FastQC (version 0.11.5)