FastQCFastQC Report
Thu 12 May 2022
EGAF00005418417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28735948
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC1021670.3555372525033801No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT607530.2114181164303332No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT586930.2042493952174468No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT567990.19765834765569593TruSeq Adapter, Index 18 (97% over 40bp)
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA455830.15862709662475724No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA415300.144522811636491No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT345400.12019787897723087No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT326700.11369035049757188No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA287910.10019157885447175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)