FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16296111
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC447230.274439711413355No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT262150.16086660185365698No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA251640.15441721033932573No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG230250.14129137927447843No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA209000.12825145827737675No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT207840.12753963200177024No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT190170.11669655416559202No Hit
TGGTTTTATGAAAGGAAGTGTTTAATTTTATTGAGTTGAATGTAAATATT185010.11353015452582522No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA180560.11079944165819686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC249050.0114.557881
CGGTTAA992050.0113.6000441
CGGGTGC80400.0113.1144561
CGGGTAC95650.0110.171061
GGGCGCG300450.0103.6965262
CGGCTAA8400.099.710011
GGCGCGG309450.097.0455253
GCGCGGT307100.095.85614
CGCGGTG312000.095.5310065
CGGAATA144050.094.267481
CGGGTAT281650.094.1273961
CGGATAC11800.093.63321
CGGGTGT252650.092.211951
CGGGCGT364900.092.1548541
CGGTAAT97400.091.968961
CGGGTTT708450.091.762631
GCGGTGG459000.089.937416
CGGATGC10700.089.9352951
CGGAATT157850.089.413021
CGGTGGT570250.088.7343147
Produced by FastQC (version 0.11.5)