FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16263810
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC570880.351012462639443No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC527720.32447501538692347TruSeq Adapter, Index 12 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT348510.21428558252955487No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT223050.1371449863223931No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT206110.12672922273440232No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT168310.10348743621574526No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA166980.10266966965305178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC268400.0114.776931
CGGTTAA1150650.0113.746871
CGGGTGC82300.0112.5334851
CGGGTAC102350.0108.423251
CGGCTAA7950.0105.701841
GGGCGCG316150.0102.6937562
GGCGCGG327000.096.489533
CGCGGTG333050.095.1476445
GCGCGGT329900.093.5672764
CGGGCGT392400.092.905211
CGGGTGT271000.091.617961
CGGGTAT315700.091.614941
CGGGTTT840450.090.9940641
CGGAATA148600.090.399431
CGGAAAT85550.090.284991
CGGATGC11550.089.7837751
GCGGTGG482600.088.3724756
CGGTGGT598300.088.228497
CGGTAAT98650.087.720311
CGGATAA83950.086.89431
Produced by FastQC (version 0.11.5)