FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11284758
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT402660.3568175764159054No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA344770.3055182929044646No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT278640.24691712485106015No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC277650.24603983532478055No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA255650.22654451251856708No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT237980.21088622370103108No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA211860.1877399586238358No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA153660.1361659682910347No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT148260.13138075269314592No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA145270.1287311610935742No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA132390.11731753574157283No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA130830.11593514012440498No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA125860.11153096947227402No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG123410.10935989943249115No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAA123050.10904088505929857No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAG122410.10847374839584509No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT120690.10694956861281384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC65300.0112.139351
CGGTTAA439950.0110.308021
CGGCTAA4050.0107.3957751
CGGGCGC155800.0105.703631
CGGGTAC63300.0104.293051
CGGGTGT191500.095.45661
CGGGAGG737100.094.680611
CGGGTAT207000.094.554971
GGGAGGC319700.093.789492
CGGAATA101650.091.5573041
GGGCGCG192300.091.5552142
CGGGTTT397050.090.157951
CGGTAAT64300.089.883521
CGGGCGT263150.088.032331
CGCGGTG193050.086.127895
CGGGTAA51700.086.089341
CGGATAC6700.085.372061
GCGCGGT196850.084.404834
GGCGCGG200900.084.124623
CGGAATG348150.083.8588641
Produced by FastQC (version 0.11.5)