FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13065507
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT553070.4233054254993702No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA369320.2826679439228803No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA352510.26980200615253586No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT339200.2596148775550769No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT261710.20030604246739142No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC247930.18975918806671643No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT247110.18913158134621183No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA209110.160047367469169No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT208070.15925137845779733No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA195180.14938570696108464No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA192980.14770188405241375No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA163350.1250238509688143No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA161110.12330941309816756No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA150320.1150510271051862No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT135630.10380768231956095No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT133810.10241470154966048No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA131580.10070791741950771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC51450.0110.8225251
CGGTTAA436600.0110.311311
CGGGCGC141150.0106.638951
CGGGTAC55850.0104.4361341
CGGCTAA3900.0100.722681
GGGAGGC416350.099.7374042
CGGGAGG869400.099.217111
CGGAATA139400.094.784671
CGGGTGT181950.094.6987841
CGGTAAT88150.094.661551
CGGATGC6100.093.6675951
CGGGTAT193900.092.8222051
GGGCGCG178250.092.590892
TAAGTCC454.0563464E-1092.583471
GCGGAGT273550.091.005567
CGGGTAA59650.089.800751
CGGGAAG120450.089.7339861
CGGATAC5000.089.276911
CGGTATT210450.088.011331
CGGGTTT360100.087.3348541
Produced by FastQC (version 0.11.5)