FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19809935
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC617000.3114598811152081No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT353080.17823380036330255No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT342320.1728021823393161No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA279490.1410857733758339No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA269380.1359822735410288No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT259030.13075762237483365No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT211160.10659297973466345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC320850.0114.8788451
CGGGTGC104750.0113.808421
CGGTTAA1313900.0113.4028851
CGGGTAC126550.0107.083431
CGGCTAA9850.0102.669951
GGGCGCG386300.0101.519532
GGCGCGG399100.094.4782643
CGGAATA198400.093.939761
CGGGTAT364150.093.5572741
CGCGGTG403850.093.396255
CGGGCGT473350.093.024511
GCGCGGT399550.092.5558554
CGGGTGT328800.092.28991
CGGATAC15200.091.971861
CGGGTTT901250.091.293311
CGGTAAT127700.091.1189351
CGGATGC13800.089.232261
CGGAATT219900.089.0564351
CGGTGGT719450.087.980337
GCGGTGG587800.087.4798056
Produced by FastQC (version 0.11.5)