FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005418479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23671977
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC820610.34665883631096805No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT475730.2009675828934778No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC425720.1798413372909242TruSeq Adapter, Index 7 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT358370.15138997473679533No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA303660.1282782591416002No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT281970.11911552634577163No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA269560.11387304068435011No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA250560.1058466726289908No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA244120.10312615629864798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)