FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19637332
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC616730.3140599751534475No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT497060.2531199248451877No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT492930.2510167878202599No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA395980.20164653731983548No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA382110.19458345970827404No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT278040.1415874620849716No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT276760.14093564237748793No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA244060.12428368578786568No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA217610.11081444261369111No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG207330.10557951558796276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC282900.0115.443121
CGGTTAA1607750.0114.958711
CGGGTGC94500.0112.029151
CGGCTAA11400.0107.0128561
CGGGTAC112550.0106.435181
GGGCGCG338550.0101.933822
CGGAATA292350.097.360821
GGCGCGG352250.095.2173
CGCGGTG356800.093.726035
GCGCGGT357700.092.043714
CGGATAC18450.091.925371
CGGGTAT358150.090.8885651
CGGGTGT319500.090.447111
CGGAAAT115400.090.295731
CGGGTTT1033100.088.996521
CGGAGTG128800.088.8947451
CGGAATT273650.088.42171
CGGATGC12350.088.180181
CGGGTAA96700.087.756641
CGGGCGT464700.087.490741
Produced by FastQC (version 0.11.5)