FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20126032
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC631460.31375285500887606No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT449900.2235413319426303No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA275630.136951983381523No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT262670.1305125620390547No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT249610.12402345380351179No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA228040.11330599096732033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1538700.0114.3848341
CGGGCGC293450.0114.322981
CGGGTGC99100.0113.162681
CGGCTAA9850.0109.926071
CGGGTAC121950.0109.716811
GGGCGCG353000.0100.478982
GGCGCGG367000.093.539073
CGGGTTT1163800.093.0171661
CGCGGTG369900.092.702355
CGGGTAT403150.092.541311
CGGAATA210800.091.892351
GCGCGGT366250.091.85634
CGGGTGT355200.091.6513061
CGGAAAT113800.090.755511
CGGAATT264500.089.633061
CGGAGTG126500.089.309981
CGGATAA102200.088.831951
CGGATAC17450.088.3018651
CGGGCGT521250.087.861251
CGGGTAA106950.087.7235951
Produced by FastQC (version 0.11.5)