FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18895125
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT342180.18109432988667712No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC327870.17352094786353625No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT273580.1447886690350024No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA237070.1254662247537394No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA234290.12399494578628086No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA213230.11284921375222445No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT196890.10420148054061565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC82400.0109.425681
CGGCTAA5500.0109.148951
CGGTTAA595600.0106.979611
CGGGCGC207050.0104.866141
CGGGTAC76950.0101.49571
CTTCCGA2400.095.7085656
GGGAGGC486550.095.692732
CGGTCTA1000.095.100081
CGGGAGG1089900.093.6036451
CGGATGC9950.093.1885151
CGGGCAT3200.092.871171
CGGCTAT900.092.458411
CGGAATA181250.092.312671
CGGGTGT298200.091.80731
CGGGTAA99600.091.4836961
CGGTAAT124000.090.881931
GGGCGCG266250.088.784892
CGGGTTT644700.088.5247961
TCCGATC2600.088.346148
CGGGTAT301150.088.223761
Produced by FastQC (version 0.11.5)