FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16879985
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC388590.23020755053988498No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA262430.1554681476316478No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG235350.13942547934728616No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT211010.12500603525417825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC240950.0112.954541
CGGTTAA917400.0112.6044541
CGGGTGC82450.0111.042051
CGGGTAC96800.0108.715641
GGGCGCG290000.0101.7799452
GGCGCGG300600.094.607773
CGCGGTG300250.094.1429755
GCGCGGT295950.094.1239854
CGGGTAT328500.093.716161
CGGGCGT394750.092.7115251
CGGGTTT828800.091.129021
CGGAAAT86050.090.634041
CGGATAC15250.090.501891
CGGAATT176250.090.491381
CGGGTGT283950.090.339241
CGGATAA74600.089.951791
CGGAATA109800.088.583921
CGGTAAT106950.088.163341
CGGAGTG89700.086.481791
CGGATGC11400.085.581231
Produced by FastQC (version 0.11.5)