FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005418511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32200855
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC896330.2783559629084383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC863090.26803325563870894TruSeq Adapter, Index 12 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA754900.23443476889045337No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT686880.21331110618025514No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG664160.20625539290804545No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA525110.16307330969938533No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT468150.14538433839722578No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT409220.12708358209743187No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA371360.11532613031548385No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA336600.10453138589021937No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA325210.10099421273130792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)