FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005418516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17455225
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC706490.4047441382164939No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT374000.214262491603517No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT255500.14637450963823154No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT205800.11790165981819198No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT205390.11766677312953572No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA202980.11628609771572695No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC200110.11464189089513313TruSeq Adapter, Index 12 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA194310.11131910359219088No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA177040.10142521795049907No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)