FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10477222
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA366840.3501309793760216No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC283200.2703006579415803No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT249580.2382119993257755No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT230650.2201442328892143No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG207400.19795323607727316No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA197020.18804602975865167No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT196060.18712975634190054No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA194920.1860416816595086No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA164450.15695954519241836No Hit
TGGTTAGTGGGGGTAGGTTTTTTAGGGAGAGGAGGGTGGATGGAATTAAG157290.15012567262581628No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT150570.14371175870855843No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC147050.14035208951380432TruSeq Adapter, Index 12 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT139920.1335468504914757No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA125420.11970730409263067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC160650.0112.8497541
CGGGTGC49400.0111.351711
CGGTTAA422200.0111.203871
CGGGTAC64300.0110.666321
CGGCTAA3800.0103.510041
GGGCGCG190000.0101.332892
CGGGTAT208500.096.583791
GGGAGGC302700.095.535472
CGGGTGT181750.095.190751
CGGGAGG700850.094.932941
CGGGTTT398650.093.210931
GGCGCGG199350.092.6413043
CGCGGTG196950.092.621885
CGGGCGT271050.092.5227361
GCGCGGT196650.092.067084
CGGTAAT63150.090.126431
CGGAATA106500.088.7514951
CGGATGC6150.088.1837161
CGGGTAA49400.087.82671
CGGAATG504750.087.1959461
Produced by FastQC (version 0.11.5)