FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15727193
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC489750.3114033127208396No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT226730.14416431463643894No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA189450.1204601482286127No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA159580.10146756639916607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA916300.0111.759291
CGGGCGC298900.0111.667531
CGGGTGC108450.0111.5864261
CGGGTAC126100.0107.480231
GGGCGCG349000.0100.382572
CGGGTAT380300.094.94691
CGGGTGT331000.094.241421
CGCGGTG354550.093.391355
GGCGCGG362700.093.378143
GCGCGGT355850.092.799454
CGGGCGT464850.092.1402361
CGGGTTT929500.091.3471451
CGGTAAT106000.087.616731
CGGTGGT665450.087.493297
CGGAATA120700.086.5087051
GCGGTGG545350.084.906726
CGGAATT175650.084.54471
CGGAGTG88100.084.28081
CGGATAA72150.083.6164251
CGGAGTA125450.083.517721
Produced by FastQC (version 0.11.5)