FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17041241
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT445160.2612251067865304No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC330200.19376523106503804No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA328900.1930023758246245No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA263000.1543314832528922No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT251990.14787068617831295No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT220140.12918073278818135No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT175930.10323778649688715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA674000.0110.9936141
CGGGTGC72900.0108.832811
CGGGCGC202850.0103.722171
CGGGTAC74800.098.509061
GGGAGGC451350.096.0080642
CGGGAGG941300.095.763141
CGGTAAT93100.094.6170041
CGGCTAA7300.093.763141
CGGGTGT224850.093.7325061
GGGCGCG256650.091.44632
CGGAATA134000.091.3220141
CGGGTAT247600.091.298061
CGGGTTT499550.089.4783861
CGGGTAA70000.089.4488751
CGGGCGT320800.086.6256941
CGGAAAT74950.086.558961
CGGGAAG138000.086.0872041
CGCGGTG263950.085.8818055
GCGGAGT287400.085.768157
CGGAATT153250.085.443571
Produced by FastQC (version 0.11.5)