FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10724740
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC322360.30057605126091635TruSeq Adapter, Index 12 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC203280.18954305652164996No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA190500.17762668372380122No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT178860.1667732737576855No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA127240.1186415707979867No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT117070.10915882343068457No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT109090.10171808360855368No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG107430.1001702605377846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC54200.0111.196751
CGGCTAA4550.0107.328071
CGGTTAA369000.0106.7775651
CGGGCGC128350.0105.744871
CGGGTAC50700.0100.313821
CGGGCAT2250.095.2863851
CGGGTGT172050.094.1164251
CGGGAGG579350.092.987891
GGGAGGC262900.092.945482
GGGCGCG166300.092.867222
CGGTAAT67900.091.479841
CGGGGAC12600.090.748941
CGGGTAT178700.090.147481
CGGAATA105900.089.584051
CGGGTTT357700.088.8398361
CGGGTAA54300.086.972951
CGGATGC5850.086.531441
CGGGAAG95150.084.6211241
GCGGAGT175950.084.2891547
CGGGAAA45800.084.2597961
Produced by FastQC (version 0.11.5)