FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18460763
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT485230.26284395720805254No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC479650.2598213302451258No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA350370.18979172204312464No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT251970.13648948312699752No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT200230.10846247254244042No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA196240.10630113175712184No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA195540.10592194916320631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC102750.0112.647081
CGGTTAA761750.0109.78921
CGGGCGC267550.0109.1551
CGGGTAC109050.0105.37411
CGGCTAA6700.0104.079111
CGGGAGG1451350.096.4512561
GGGAGGC621550.096.036112
CGGGTGT360350.095.616081
CGGGTAT401000.093.830481
CGGTAAT149100.093.338711
CGGGCAT3450.093.28831
GGGCGCG333650.093.0285342
CGGGTTT790200.090.9022451
CGGAATA219450.090.2228551
CGGGTAA118100.090.183521
CGGGAAG224550.088.492211
CGGTATT378950.087.793111
CGGTATA124350.087.1366041
GCGGAGT395000.086.811797
CGGGATA112650.086.769121
Produced by FastQC (version 0.11.5)