FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15798231
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC602460.381346493794147No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT172170.1089805561141624No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT163580.10354323848030833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC319950.0114.0715561
CGGGTGC95000.0113.695121
CGGTTAA653900.0110.197681
CGGGTAC119200.0109.848681
GGGCGCG387700.0102.87422
CGGCTAA5750.097.8891451
CGGGTAT350500.096.809491
CGCGGTG395400.096.738965
GCGCGGT392700.096.23054
GGCGCGG402450.095.8569263
CGGGTGT291400.093.895141
CGGGCGT471150.093.397011
CGGTAAT113650.091.857631
GCGGTGG559900.090.74446
GGGAGGC376850.090.696432
CGGTGGT696300.090.161777
CGGGTTT585300.089.353871
CGGGAGG905100.088.869771
CGGATGC10700.088.5961761
CGGATAC14600.088.061421
Produced by FastQC (version 0.11.5)