FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7945019
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT345380.43471261679802153No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC295330.3717171727342628No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA246270.30996779239923783No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT192150.2418496418951295No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT189550.23857715129441479TruSeq Adapter, Index 18 (97% over 40bp)
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA137190.17267422519694414No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT114720.14439235450538257No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT93020.11707964449172495No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA89760.11297644473852109No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA88220.11103812338271311No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA85910.10813064134900117No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT83900.10560075438460247No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT81840.10300793490865157No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT80900.1018248036914701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC148450.0114.223431
CGGGTGC50000.0113.281351
CGGTTAA345000.0111.522211
CGGGTAC59250.0109.970671
GGGCGCG175700.0102.111612
CGGCTAA2850.0100.310011
CGGGTAT168000.097.5279851
CGGGTGT146850.096.5274661
CGGGAGG581200.094.359931
CGCGGTG184350.094.188145
GGGAGGC251100.093.868022
CGGGTTT316050.093.7530061
GGCGCGG186200.093.667783
GCGCGGT185550.092.3282854
CGGAATA114250.092.270941
CGGTAAT52250.091.988841
CGGGCGT215550.091.486941
CGGATAC5450.090.7046361
CGGGTAA43700.088.997881
CGGAAAT43500.088.9963151
Produced by FastQC (version 0.11.5)