FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005418578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20576632
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT745360.36223615215551314No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC532560.25881786679180535No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA502860.24438401775373153No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT411420.19994525829105558No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA340060.1652651415450303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC311040.1511617644714645TruSeq Adapter, Index 2 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT250000.12149704577503256No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA220190.1070097380368177No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT214390.10419100657483693No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT209260.10169788719553326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)