FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23300353
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC790210.339140784691116No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT616180.26445092913399204No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA434220.18635769166243962No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT349550.15001918640460082No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA334040.14336263489226966No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA277320.11901965605413789No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT250280.10741468165739808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC139950.0112.658861
CGGGCGC428550.0111.461971
CGGTTAA1023100.0110.712551
CGGGTAC170100.0108.33561
CGGCTAA8300.0105.4536741
GGGCGCG518600.0100.090452
CGGGTAT486650.096.95171
CGGGTGT407300.095.7877961
CGGAATA314650.095.085661
CGGGCGT611200.093.876311
GGGAGGC666300.093.861952
CGGGAGG1503600.093.853961
CGGTAAT181650.093.6010061
GGCGCGG539900.092.866513
CGCGGTG539600.092.463825
GCGCGGT532750.092.214624
CGGATGC15000.091.863451
CGGGTAA122200.089.5320741
CGGGAAG233750.089.5126341
CGGATAC19700.089.1596451
Produced by FastQC (version 0.11.5)