FastQCFastQC Report
Thu 12 May 2022
EGAF00005418607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28806736
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC1098350.38128235007256633No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC738470.25635323627085TruSeq Adapter, Index 4 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT624650.21684164425987032No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT537390.18655011800017884No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA422470.14665667085642747No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT385450.13380550993350998No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT374220.1299071161689405No Hit
TGGTTTTATGAAAGGAAGTGTTTAATTTTATTGAGTTGAATGTAAATATT370950.12877196500151908No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT315460.10950910925833458No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT295860.10270514507440204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)