FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12161882
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC530420.43613315768069444No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA225340.18528382367136928No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT211660.17403556456147165TruSeq Adapter, Index 13 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT198770.16343687597034734No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA150820.12401041220429536No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT143030.11760515354449254No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT125950.10356127448038059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC253650.0113.8111341
CGGGTGC77050.0112.415951
CGGTTAA550750.0111.884571
CGGGTAC97550.0107.783821
GGGCGCG298900.0102.4134142
CGGGTAT277100.097.407791
GCGCGGT304450.095.413574
CGCGGTG307800.095.3414845
GGCGCGG311400.095.0034263
CGGGTGT236600.094.216111
CGGGCGT384250.091.5155941
CGGGTTT517750.091.275361
CGGCTAA4400.090.7106551
CGGTGGT531450.089.962247
CGGAATA109350.089.94241
GGGAGGC321800.089.706092
GCGGTGG437500.088.925936
CGGGAGG777400.088.713081
CGGATAC11550.087.680521
CGGTAAT79300.087.6665341
Produced by FastQC (version 0.11.5)