FastQCFastQC Report
Thu 12 May 2022
EGAF00005418617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28543961
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC865390.3031779646840184No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT637370.2232941671970474TruSeq Adapter, Index 14 (97% over 44bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT515740.18068270202583306No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT477960.16744697766368166No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA473260.16580039469644736No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA408160.14299346891624465No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG380880.13343628097025498No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA373580.13087882231901873No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT360520.12630342369091663No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA359370.12590053636914653No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT297360.10417615130570002No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT288800.10117726828452435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)