FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005418621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12587442
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC282680.22457303080324026No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT201820.16033440312972247TruSeq Adapter, Index 16 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT157450.12508498549586167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)