FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14692140
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT431100.29342219717481594No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC351160.23901215207587184No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA292230.19890227019345036No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT229630.15629445404141262No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA184170.12535273962812768No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT166650.11342799619388326No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC164310.1118353078584876TruSeq Adapter, Index 12 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA147740.10055716866297217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC66850.0112.173221
CGGTTAA470550.0110.44041
CGGGCGC182150.0110.173821
CGGGTAC71200.0109.499291
CGGCTAA3350.0103.0393751
GGGAGGC495800.097.925482
CGGGAGG1041750.097.787691
GGGCGCG226200.096.7430042
CGGATGC6150.095.8032151
CGGGTGT227950.095.530671
CGGGTAT261350.095.208931
CGGTAAT92850.092.492051
CGGGTAA75350.091.305011
GCGGAGT321600.090.867987
CGGGCGT314900.089.7342151
CGGATAC8900.089.605531
GGCGCGG239250.088.7581253
CGGAATA160250.088.3520051
CGCGGTG240750.087.9799965
CGGGTTT433200.087.911091
Produced by FastQC (version 0.11.5)