FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18631632
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC433080.2324434059238611No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT268030.14385749997638425No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA241540.12963974385067287No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG225470.12101462716738931No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA202100.10847144254459298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1163000.0113.777751
CGGGCGC286850.0111.646361
CGGGTGC99950.0111.014631
CGGGTAC116850.0107.6435241
GGGCGCG342850.0100.881542
CGGGCGT389250.095.305061
GGCGCGG353600.094.802063
CGGGTAT315950.094.392281
CGGGTGT280400.093.79181
CGGAATA169850.092.909761
CGCGGTG360100.092.641325
GCGCGGT354800.092.1515664
CGGTAAT115050.091.3736951
CGGGTTT756400.089.062921
CGGATAA84100.088.901851
CGGAATT199900.087.6384351
CGGTGGT676050.086.667967
CGGAAAT109350.086.61011
CGGATAC15750.085.795091
GCGGTGG546900.085.5361256
Produced by FastQC (version 0.11.5)