FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19276780
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC672070.34864225249237685No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT383620.19900626556924964No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA316800.16434279999045484No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG278840.14465071448654807No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT265400.13767859569907423TruSeq Adapter, Index 13 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT229500.11905515340217608No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA227220.11787238325072964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC331200.0113.939081
CGGTTAA1304850.0113.5808641
CGGGTGC99900.0112.8828351
CGGGTAC123450.0107.576681
CGGCTAA10050.0107.06281
GGGCGCG395650.0101.670052
GGCGCGG409150.095.7223743
CGCGGTG410400.094.808535
GCGCGGT410800.093.227084
CGGGTAT369350.092.368571
CGGATAC18550.091.9737851
CGGGTGT330300.091.662391
CGGAATA171500.090.920181
CGGAAAT107200.090.777871
CGGGCGT498950.090.620281
CGGGTTT973400.090.446081
CGGAATT231000.088.397671
CGGTGGT710450.088.275477
CGGTAAT123300.087.9884951
CGGATAA100050.087.8773961
Produced by FastQC (version 0.11.5)